mTOR Plays a Conserved Role in Regulation of Nutritional Metabolism in Bivalve Sinonovacula constricta

The mammalian target of rapamycin (mTOR) has been shown to play a central role in regulating cell growth and metabolism. However, little is known about the function mTOR nutrient metabolism bivalve mollusks. In this study, regulation was investigated Sinonovacula constricta. First, activation assayed after starvation refeeding. Afterwards, using an activator (MHY1485) or inhibitor (rapamycin) mTOR. open reading frame S. constricta 7416 bp length encodes polypeptide consisting 2471 amino acids. acid sequence highly conserved when compared with other species had close evolutionary relationship TOR proteins Crassostrea gigas Lingula anatine. expressed intestine, exhalent siphon, labial palppus, muscle, inhalent gill, mantle, digestive land, gonad tissue constricta, highest expression muscle. During starvation, level phosphorylated protein relatively low, ratio LC3II/LC3I AMPKα mRNA significantly increased increase time. After feeding, from 0.13 0.56, LC3II/I decreased 1.17 0.38. MHY1485 4E-BP1 proteins. Furthermore, glucose metabolism-related gene glucokinase (GK), G6P gene, lipid synthesis-related genes sterol-regulatory element-binding (SREBP) stearoyl-CoA desaturase (SCD). Rapamycin reduced mTOR, 4EBP1 0.97 0.28. Meanwhile, it also GK, pyruvate kinase (PK), transporter 1 (GLUT1), G6P, as well SCD acetyl-CoA carboxylase (ACC). These results indicate nutritional metabolism, including synthesis, autophagy